Description
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-ANG II antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-ANG II antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ANG II amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ANG II can be calculated.
Background: Angiopoietins are protein growth factors that promote angiogenesis, the formation of blood vessels from pre-existing blood vessels. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. ANG II is a naturally occurring antagonist of angiopoietin-1 (ANGPT1) that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. ANG II is a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema. It plays a critical role in inducing tumor cell infiltration, and that this invasive phenotype is caused by activation of MMP2